Humanize Antibody - BioPhi Antibody design platform

Humanize an antibody using deep learning on natural antibody repertoires (Sapiens) or CDR grafting.

1Input sequences

Input mode

Single sequence Bulk input

Name (optional)

Antibody heavy chain sequence

Use example

Antibody light chain sequence

Use example

Provide multiple antibody sequences. Both chains of an antibody should have the same ID in the FASTA header, optionally with _HC/_LC or _VH/_VL suffix.

Antibody sequences (FASTA)

Use example

Upload file (FASTA/PDB)

PDB IDs

Use example

2Humanization settings

Numbering

CDR Definition

Humanization method

Sapiens CDR Grafting

Sapiens is a high-throughput humanization method based on deep learning on antibody repertoires of more than 500 human subjects from the Observed Antibody Space database.

Humanization iterations

Humanize CDRs

Graft the CDR regions into the nearest human V and J gene sequences based on % sequence identity.

Heavy germline

Light germline

Perform final humanization pass using Sapiens

3Humanness evaluation settings

OASis prevalence threshold

loose (≥1% subjects)

relaxed (≥10% subjects)

medium (≥50% subjects)

strict (≥90% subjects)

custom

% subjects

4Submit

Submit the task to the queue. Results are stored for a 24-hour period and accessible under a secret URL.