Humanness - BioPhi Antibody design platform

Evaluate antibody humanness using peptide search in natural antibody repertoires (OASis) and germline sequence identity.

1Input sequences

Input mode

Single sequence Bulk input

Name (optional)

Antibody heavy chain sequence

Use example

Antibody light chain sequence

Use example

Provide multiple antibody sequences. Both chains of an antibody should have the same ID in the FASTA header, optionally with _HC/_LC or _VH/_VL suffix.

Antibody sequences (FASTA)

Use example

Upload file (FASTA/PDB)

PDB IDs

Use example

2Humanness evaluation settings

Numbering

CDR Definition

OASis prevalence threshold

loose (≥1% subjects)

relaxed (≥10% subjects)

medium (≥50% subjects)

strict (≥90% subjects)

custom

% subjects

4Submit

Submit the task to the queue. Results are stored for a 24-hour period and accessible under a secret URL.

Humanness report

1Input sequences

2Humanness evaluation settings

4Submit